Evaluating The Expression of Self-Renewal Genes in Human Endothelial Progenitor Cells
نویسندگان
چکیده
OBJECTIVE Endothelial progenitor cells (EPCs) have a potential application for cell therapy, however, their biological nature is not well-understood. EPCs also possess some stemness features, such as their clonogenicity and differentiation capacity. The main aim of this study was to evaluate the expression of certain transcription factors regulating selfrenewal property of stem cells. MATERIALS AND METHODS In this experimental study, peripheral blood mononuclear cells were isolated from fresh human blood of several volunteers and were cultured in fibronectin- coated plates. EPCs were identified based on their morphology and growth characteristic. Then, the expression of some markers implicated in self-renewal capacity was assessed in the isolated cells using reverse transcription-polymerase chain reaction (RTPCR) and immunocytochemistry. RESULTS Expression of the cell surface markers, CD31 and CD34, was determined by RT-PCR and immunocytochemistry. Furthermore, these cells had the ability for Di-ACLDL incorporation as well as attachment to lectin I. EPCs did not express the main stem cell markers, like OCT4-A, Nanog, and Sox2; nevertheless, they expressed the weaker pluripotent markers, including OCT4B and OCT4-B1 spliced variants, such as Nucleostemin and ZFX. Furthermore, the expression of Nucleostemin and ZFX genes revealed a decreasing pattern from days 4(th) to 11(th). CONCLUSION The main regulators of stem cell self-renewal genes, including OCT4-A, Nanog, and Sox2 are not expressed in EPCs. Forced expression of these genes can elevate the stemness property and clinical application of EPCs.
منابع مشابه
Isolation, Induction of Neural and Glial Differentiation and Evaluating the Expression of Five Self Renewal Genes in Adult Mouse Neural Stem Cells
Purpose: Isolation, induction of neural and glial differentiation and evaluating the expression of Nucleostemin, ZFX, Hoxb-4, Sox-9 & Bmi-1 self renewal genes in adult mouse neural stem cells. Materials and Methods: Breifly, for isolation of neural stem cells, frontal part of adult mouse brain was minced in PBS and digested by enzyme solution, containing hyaloronidase and trypsin. Isolated cel...
متن کاملResponse of Endothelial Progenitor Cells and Expression of Angiogenic Cytokine Genes in Preconditioning with Resistance Training in Elderly Men
Introduction: Impairment in the endothelial function starts with aging; however, it can be reduced or prevented by regular exercise. This study aimed to investigate the response and compatibility of endothelial progenitor cells and to evaluate the gene expression of hypoxia-inducible factor-1 (HIF-1) and vascular endothelial growth factor (VEGF) after eight weeks of resistance training in the e...
متن کاملChronic Exposure of Human Endothelial Progenitor Cells to Diabetic Condition Abolished the Regulated Kinetics Activity of Exosomes
By virtue of lifestyle change, incidence of type 2 diabetes is increasingly being raised with different up-surging pathologies. This condition found to disqualify endothelial progenitor cells during neo-vascularization. Besides to an aborted differentiation property, malfunctioned paracrine activities exacerbate vascular abnormalities. It is found nano-scaled exosomes play essential roles on re...
متن کاملChronic Exposure of Human Endothelial Progenitor Cells to Diabetic Condition Abolished the Regulated Kinetics Activity of Exosomes
By virtue of lifestyle change, incidence of type 2 diabetes is increasingly being raised with different up-surging pathologies. This condition found to disqualify endothelial progenitor cells during neo-vascularization. Besides to an aborted differentiation property, malfunctioned paracrine activities exacerbate vascular abnormalities. It is found nano-scaled exosomes play essential roles on re...
متن کاملDeregulation of Stemness-Related Genes in Endometriotic Mesenchymal Stem Cells: Further Evidence for Self-Renewal/Differentiation Imbalance
Background: Any irregularities in self-renewal/differentiation balance in endometriotic MSCs can change their fate and function, resulting in endometriosis development. This study aimed to evaluate the expression of OCT4 transcripts (OCT4A, OCT4B, and OCT4B1), SOX2, and NANOG in endometriotic MSCs to show their aberrant expression and to support self-renewal/differentiation imbalance in these c...
متن کامل